IGF2R Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: IGF2R Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-750
• Supplier Catalog Number: 14-750
• Alternative Catalog Number: PRS-14-750-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human IGF2R (NP_000867.2).
• Conjugation: Unconjugated
• Alternative Names: IGF2R, CD222 antigen, CIMPR, CI Man-6-P receptor, CI-MPR, IGF-II receptor, MPR 300, MPR1, MPRI, M6P-R, M6P/IGF2R, CD222, M6P/IGF2 receptor

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 310kDa
• NCBI: 3482
• UniProt: P11717
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of HeLa cells, using IGF2R antibody (14-750) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human placenta using IGF2R antibody (14-750) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using IGF2R antibody (14-750) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using IGF2R antibody (14-750) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using IGF2R antibody (14-750) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using IGF2R antibody (14-750) at dilution of 1:100. Blue: DAPI for nuclear staining.