AK1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: AK1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-15-002
• Supplier Catalog Number: 15-002
• Alternative Catalog Number: PRS-15-002-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).
• Conjugation: Unconjugated
• Alternative Names: AK1, HTL-S-58j

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 22kDa
• NCBI: 203
• UniProt: P00568
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using AK1 antibody (15-002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat lung using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using AK1 antibody (15-002). Blue: DAPI for nuclear staining.