COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-15-039
Article Name: COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-15-039
Supplier Catalog Number: 15-039
Alternative Catalog Number: PRS-15-039-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human COL1A1 (NP_000079.2).
Conjugation: Unconjugated
Alternative Names: Alpha-1 type I collagenOI4
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 139kDa
NCBI: 1277
UniProt: P02452
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using COL1A1 antibody (15-039) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human colon carcinoma using COL1A1 antibody (15-039) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COL1A1 antibody (15-039) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 antibody (15-039) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of rat ovary using COL1A1 antibody (15-039) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human breast cancer using COL1A1 antibody (15-039) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human breast cancer using COL1A1 antibody (15-039) at dilution of 1:100. Blue: DAPI for nuclear staining.