CAMLG Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-15-165
Article Name: CAMLG Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-15-165
Supplier Catalog Number: 15-165
Alternative Catalog Number: PRS-15-165-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CAMLG (NP_001736.1).
Conjugation: Unconjugated
Alternative Names: CAMLG, CAML, MGC163197
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 38kDa
NCBI: 819
UniProt: P49069
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CAMLG antibody (15-165) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CAMLG Polyclonal Antibody (15-165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CAMLG Polyclonal Antibody (15-165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.