S100A8 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: S100A8 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-15-986
• Supplier Catalog Number: 15-986
• Alternative Catalog Number: PRS-15-986-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).
• Conjugation: Unconjugated
• Alternative Names: 60B8AG, CAGA, calgranulin A, calgranulin-A, calprotectin L1L subunit, CFAG, CGLA, CP-10, cystic fibrosis antigen, L1Ag, leukocyte L1 complex light chain, MA387, MIF, migration inhibitory factor-related protein 8, MRP8, MRP-8, NIF, OTTHUMP00000015329, P8, protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, urinary stone protein band A

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 11kDa
• NCBI: 6279
• UniProt: P05109
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using S100A8 antibody (15-986) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human tonsil using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human skin using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody (15-986) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (15-986) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.