Acetyl-Histone H2B-K15 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Acetyl-Histone H2B-K15 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-143
• Supplier Catalog Number: 16-143
• Alternative Catalog Number: PRS-16-143-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: AA synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B
• Conjugation: Unconjugated

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (16-143) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.