ZNRF3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-16-383
Article Name: ZNRF3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-16-383
Supplier Catalog Number: 16-383
Alternative Catalog Number: PRS-16-383-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZNRF3 (NP_115549.2).
Conjugation: Unconjugated
Alternative Names: BK747E2.3, RNF203, E3 ubiquitin-protein ligase ZNRF3, CTA-292E10.6, RING finger protein 203, RING-type E3 ubiquitin transferase ZNRF3, novel C3HC4 type Zinc finger (ring finger), zinc/RING finger protein 3
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 120kDa
NCBI: 84133
UniProt: Q9ULT6
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ZNRF3 antibody (16-383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).