Cyclin B1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Cyclin B1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-390
• Supplier Catalog Number: 16-390
• Alternative Catalog Number: PRS-16-390-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1).
• Conjugation: Unconjugated
• Alternative Names: CCNB1, CCNB

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 55kDa
• NCBI: 891
• UniProt: P14635
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (16-390) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 antibody (16-390) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 antibody (16-390) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 antibody (16-390) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 antibody (16-390) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of 293T cells using Cyclin B1 antibody (16-390) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Cyclin B1 antibody (16-390). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (16-390) at a dilition of 1:1000.