S100A4 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: S100A4 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-542
• Supplier Catalog Number: 16-542
• Alternative Catalog Number: PRS-16-542-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).
• Conjugation: Unconjugated
• Alternative Names: S100A4, S100 calcium binding protein A4, 18A2, 42A, CAPL, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, malignant transformation suppression 1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 12kDa
• NCBI: 6275
• UniProt: P26447
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using S100A4 antibody (16-542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using S100A4 antibody (16-542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded rat brain using S100A4 antibody (16-542) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using S100A4 antibody (16-542) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using S100A4 antibody (16-542) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using S100A4 antibody (16-542) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using S100A4 antibody (16-542) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using S100A4 antibody (16-542) at dilution of 1:100. Blue: DAPI for nuclear staining.