CASP3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CASP3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-751
• Supplier Catalog Number: 16-751
• Alternative Catalog Number: PRS-16-751-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein of human CASP3.
• Conjugation: Unconjugated
• Alternative Names: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 31kDa
• NCBI: 836
• UniProt: P42574
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using CASP3 antibody (16-751) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Caspase 3 antibody (16-751) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using Caspase 3 antibody (16-751) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using Caspase 3 antibody (16-751) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using Caspase 3 antibody (16-751) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using Caspase 3 antibody (16-751) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Caspase 3 antibody (16-751) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using Caspase 3 antibody (16-751) at dilution of 1:100. Blue: DAPI for nuclear staining.