MLKL Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-16-911
Article Name: MLKL Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-16-911
Supplier Catalog Number: 16-911
Alternative Catalog Number: PRS-16-911-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of mouse MLKL
Conjugation: Unconjugated
Alternative Names: MLKL
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 54kDa
NCBI: 197259
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MLKL antibody (16-911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using MLKL antibody (16-911) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using MLKL antibody (16-911) at dilution of 1:200 (40x lens).