Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:1000IHC: 1:50 - 1:200
Western blot analysis of extracts of BT-474 cells, using TFF1 antibody (18-153) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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