TMPRSS2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-426
Article Name: TMPRSS2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-426
Supplier Catalog Number: 18-426
Alternative Catalog Number: PRS-18-426-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-400 of human TMPRSS2 (NP_001128571.1).
Conjugation: Unconjugated
Alternative Names: transmembrane protease, serine 2, PRSS10, epitheliasin, TMPS2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 52kDa
NCBI: 7113
UniProt: O15393
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of 22Rv1 cells, using TMPRSS2 antibody (18-426) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach cancer using TMPRSS2 antibody (18-426) at dilution of 1:200 (40x lens).