HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-488
• Supplier Catalog Number: 18-488
• Alternative Catalog Number: PRS-18-488-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3).
• Conjugation: Unconjugated
• Alternative Names: HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 60kDa
• NCBI: 3066
• UniProt: Q92769
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using HDAC2 antibody (18-488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using HDAC2 antibody (18-488). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody (18-488). Western blot was performed from the immunoprecipitate using HDAC2 antibody (18-488) at a dilition of 1:1000.