PPP4C Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PPP4C Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-508
• Supplier Catalog Number: 18-508
• Alternative Catalog Number: PRS-18-508-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
• Conjugation: Unconjugated
• Alternative Names: PPP4C, protein phosphatase 4 (formerly X), catalytic subunit, PP4, PPH3, PPX, Protein phosphatase 4, PP4C, PPP4

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 36kDa
• NCBI: 5531
• UniProt: P60510
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using PPP4C antibody (18-508) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat liver using PPP4C antibody (18-508) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody (18-508) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using PPP4C antibody (18-508) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF-7 cells using PPP4C antibody (18-508). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody (18-508). Western blot was performed from the immunoprecipitate using PPP4C antibody (18-508) at a dilition of 1:1000.