MonoMethyl-Histone H3-K9 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MonoMethyl-Histone H3-K9 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-621
• Supplier Catalog Number: 18-621
• Alternative Catalog Number: PRS-18-621-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Other, Rat
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
• Conjugation: Unconjugated
• Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 18kDa
• NCBI: 8290
• UniProt: Q16695
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Images

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K9 antibody (18-621).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (18-621).

Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H3-K9 antibody (18-621) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human embryo brain using MonoMethyl-Histone H3-K9 antibody (18-621) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 antibody (18-621). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-K9 antibody (18-621) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 antibody (18-621) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 antibody (18-621) at dilution of 1:100. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using MonoMethyl-Histone H3-K9 antibody (18-621) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.