DiMethyl-Histone H3-K36 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DiMethyl-Histone H3-K36 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-628
• Supplier Catalog Number: 18-628
• Alternative Catalog Number: PRS-18-628-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Other, Rat
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
• Conjugation: Unconjugated
• Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 18kDa
• NCBI: 8290
• UniProt: Q16695
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Images

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody (18-628).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (18-628).

Immunohistochemistry of paraffin-embedded human breast using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody (18-628). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of gamma-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody (18-628) and rabbit IgG. P1, P2 and P3 were probes located on gamma-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.