FLNB Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: FLNB Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-661
• Supplier Catalog Number: 18-661
• Alternative Catalog Number: PRS-18-661-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).
• Conjugation: Unconjugated
• Alternative Names: FLNB, filamin B, beta (actin binding protein 278), ABP-278, AOI, DKFZp686A1668, DKFZp686O033, FH1, FLN1L, LRS1, SCT, TABP, TAP, beta filamin, filamin 1 (actin-binding protein-280)-like, beta (actin-binding protein-278), FLN3

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 298kDa
• NCBI: 2317
• UniProt: O75369
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:20 - 1:100

Images

Western blot analysis of extracts of various cell lines, using FLNB antibody (18-661) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (18-661) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (18-661) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (18-661) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HUVEC cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.