HSPB8 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HSPB8 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-681
• Supplier Catalog Number: 18-681
• Alternative Catalog Number: PRS-18-681-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
• Conjugation: Unconjugated
• Alternative Names: HSPB8, heat shock 22 kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27 kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, adult, autosomal dominant, CRYAC, PP1629

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 22kDa
• NCBI: 26353
• UniProt: Q9UJY1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using HSPB8 antibody (18-681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody (18-681) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using HSPB8 antibody (18-681). Blue: DAPI for nuclear staining.