TMPO Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: TMPO Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-696
• Supplier Catalog Number: 18-696
• Alternative Catalog Number: PRS-18-696-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
• Conjugation: Unconjugated
• Alternative Names: Thymopoietin Antibody: TP, LAP2, CMD1T, LEMD4, PRO0868, Lamina-associated polypeptide 2, isoform alpha, Thymopoietin isoform alpha, TP alpha

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 51kDa/80kDa
• NCBI: 7112
• UniProt: P42166
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using TMPO antibody (18-696) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (18-696) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TMPO antibody (18-696).