IRF2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-709
Article Name: IRF2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-709
Supplier Catalog Number: 18-709
Alternative Catalog Number: PRS-18-709-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2).
Conjugation: Unconjugated
Alternative Names: IRF2, IRF-2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 42kDa
NCBI: 3660
UniProt: P14316
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using IRF2 antibody (18-709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using IRF2 antibody (18-709). Blue: DAPI for nuclear staining.