KRT7 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-719
Article Name: KRT7 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-719
Supplier Catalog Number: 18-719
Alternative Catalog Number: PRS-18-719-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3).
Conjugation: Unconjugated
Alternative Names: Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 51kDa
NCBI: 3855
UniProt: P08729
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of HeLa cells, using KRT7 antibody (18-719).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (18-719) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.