COX5B Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: COX5B Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-748
• Supplier Catalog Number: 18-748
• Alternative Catalog Number: PRS-18-748-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
• Conjugation: Unconjugated
• Alternative Names: Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, COX5B

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 14kDa
• NCBI: 1329
• UniProt: P10606
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using COX5B antibody (18-748) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse heart using COX5B antibody (18-748) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using COX5B antibody (18-748) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug COX5B antibody (18-748). Western blot was performed from the immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000.