DDB1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DDB1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-857
• Supplier Catalog Number: 18-857
• Alternative Catalog Number: PRS-18-857-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide of human DDB1
• Conjugation: Unconjugated
• Alternative Names: DDB1, damage-specific DNA binding protein 1, 127 kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD)

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 127kDa
• NCBI: 1642
• UniProt: Q16531
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using DDB1 Antibody (18-857) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody (18-857) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody (18-857) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using DDB1 Antibody (18-857) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal Antibody (18-857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal Antibody (18-857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.