Asymmetric DiMethyl-Histone H3-R2 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Asymmetric DiMethyl-Histone H3-R2 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-967
• Supplier Catalog Number: 18-967
• Alternative Catalog Number: PRS-18-967-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Mouse, Rat
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3
• Conjugation: Unconjugated
• Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 17kDa
• NCBI: 8290
• UniProt: Q16695
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Images

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody (18-967) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody (18-967).

Immunohistochemistry of paraffin-embedded rat brain using H3R2me2a antibody (18-967) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using H3R2me2a antibody (18-967) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H3R2me2a antibody (18-967) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R2 antibody (18-967). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R2 antibody (18-967) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Asymmetric DiMethyl-Histone H3-R2 antibody (18-967) at dilution of 1:100. Blue: DAPI for nuclear staining.