Western blot analysis of extracts of various cell lines, using PVRL2 antibody (19-627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunofluorescence analysis of HeLa cells using PVRL2 antibody (19-627). Blue: DAPI for nuclear staining.
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