ATP5B Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ATP5B Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-19-846
• Supplier Catalog Number: 19-846
• Alternative Catalog Number: PRS-19-846-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2).
• Conjugation: Unconjugated
• Alternative Names: ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 57kDa
• NCBI: 506
• UniProt: P06576
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using ATP5B Antibody (19-846) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.