ATP5A1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ATP5A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-19-918
• Supplier Catalog Number: 19-918
• Alternative Catalog Number: PRS-19-918-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
• Conjugation: Unconjugated
• Alternative Names: ATP5A1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 53kDa
• NCBI: 498
• UniProt: P25705
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (19-918) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ATP5A1 antibody (19-918) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (19-918) at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (19-918) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug ATP5A1 antibody (19-918). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (19-918) at a dilition of 1:1000.