LMNB2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-257
Article Name: LMNB2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-257
Supplier Catalog Number: 22-257
Alternative Catalog Number: PRS-22-257-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 341-620 of human LMNB2 (NP_116126.3).
Conjugation: Unconjugated
Alternative Names: LMNB2, LAMB2, LMN2, MGC2721
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 70kDa
NCBI: 84823
UniProt: Q03252
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using LMNB2 antibody (22-257) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody (22-257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody (22-257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LMNB2 Antibody (22-257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using LMNB2 Antibody (22-257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using LMNB2 Antibody (22-257) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody (22-257) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using LMNB2 antibody (22-257). Blue: DAPI for nuclear staining.