DDX1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DDX1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-324
• Supplier Catalog Number: 22-324
• Alternative Catalog Number: PRS-22-324-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
• Conjugation: Unconjugated
• Alternative Names: DDX1, DBP-RB, UKVH5d

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 100kDa
• NCBI: 1653
• UniProt: Q92499
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100IP: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using DDX1 antibody (22-324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using DDX1 antibody (22-324). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody (22-324). Western blot was performed from the immunoprecipitate using DDX1 antibody (22-324) at a dilition of 1:1000.