SOX5 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SOX5 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-607
• Supplier Catalog Number: 22-607
• Alternative Catalog Number: PRS-22-607-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SOX5 (NP_694534.1).
• Conjugation: Unconjugated
• Alternative Names: SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 70kDa
• NCBI: 6660
• UniProt: P35711
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using SOX5 antibody (22-607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using SOX5 antibody (22-607).