STAT4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: STAT4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-613
• Supplier Catalog Number: 22-613
• Alternative Catalog Number: PRS-22-613-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1).
• Conjugation: Unconjugated
• Alternative Names: STAT4, SLEB11

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 76kDa
• NCBI: 6775
• UniProt: Q14765
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Images

Western blot analysis of extracts of NCI-H460 cells, using STAT4 antibody (22-613) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spleen using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectum using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectal cancer using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using STAT4 antibody (22-613) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using STAT4 antibody (22-613). Blue: DAPI for nuclear staining.