CCAR2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CCAR2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-722
• Supplier Catalog Number: 22-722
• Alternative Catalog Number: PRS-22-722-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3).
• Conjugation: Unconjugated
• Alternative Names: CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 100kDa
• NCBI: 57805
• UniProt: Q8N163
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using CCAR2 antibody (22-722) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectal cancer using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using CCAR2 antibody (22-722) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using CCAR2 antibody (22-722). Blue: DAPI for nuclear staining.