Acetyl-Histone H4-K8 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Acetyl-Histone H4-K8 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-817
• Supplier Catalog Number: 22-817
• Alternative Catalog Number: PRS-22-817-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Mouse, Rat
• Immunogen: A synthetic peptide of human Acetyl-Histone H4-K8
• Conjugation: Unconjugated
• Alternative Names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 11kDa
• NCBI: 8370
• UniProt: P62805
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:20 - 1:50CHIP seq: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (22-817) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (22-817). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (22-817) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.