Western blot analysis of extracts of various cell lines, using THOC1 antibody (23-447) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse kidney using THOC1 antibody (23-447) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using THOC1 antibody (23-447) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using THOC1 antibody (23-447) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using THOC1 antibody (23-447) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using THOC1 antibody (23-447) at dilution of 1:100. Blue: DAPI for nuclear staining.
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