TEFM Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-23-631
Article Name: TEFM Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-23-631
Supplier Catalog Number: 23-631
Alternative Catalog Number: PRS-23-631-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-210 of human TEFM (NP_078959.3).
Conjugation: Unconjugated
Alternative Names: C17orf42, transcription elongation factor, mitochondrial, transcription elongation factor of mitochondria
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 37kDa
NCBI: 79736
UniProt: Q96QE5
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using TEFM antibody (23-631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TEFM antibody (23-631) at dilution of 1:100 (40x lens).