Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using TEFM antibody (23-631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TEFM antibody (23-631) at dilution of 1:100 (40x lens).
* VAT and and shipping costs not included. Errors and price changes excepted