ELP3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-23-954
Article Name: ELP3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-23-954
Supplier Catalog Number: 23-954
Alternative Catalog Number: PRS-23-954-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 358-547 of human ELP3 (NP_060561.3).
Conjugation: Unconjugated
Alternative Names: Elongator complex protein 3, hELP3, ELP3
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 62kDa
NCBI: 55140
UniProt: Q9H9T3
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of Mouse kidney, using ELP3 antibody (23-954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.
Immunohistochemistry of paraffin-embedded human lung cancer using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ELP3 antibody (23-954) at dilution of 1:200 (40x lens).