Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:2000
Figure 1 Western Blot Validation in Human Cells and Mouse Kidney TissueLoading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, 24-030, at 1:3000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST
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