Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
Figure 2 Immunohistochemistry Validation of TMPRSS2 in Human Esophageal Tissue Immunohistochemistry of paraffin-embedded human esophageal tissue using TMPRSS2 antibody, 24-034, at 1:100 dilution (40x lens).
Figure 1 Western Blot Validation in Human Cell Lines and Mouse and Rat TissuesLoading: 25 µg of lysates per lane. Antibodies: TMPRSS2 antibody, 24-034, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
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