CD28 Antibody [C28/75], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: CD28 Antibody [C28/75], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-33-091
• Supplier Catalog Number: 33-091
• Alternative Catalog Number: PRS-33-091-100
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: FA, FC, IHC, IP
• Species Reactivity: Human
• Immunogen: Recombinant human CD28 protein was used as the immunogen for this antibody.
• Conjugation: Unconjugated
• Alternative Names: T-cell-specific surface glycoprotein CD28, TP44, CD28, CD28

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL
• Clone Designation: [C28/75]
• NCBI: 940
• UniProt: P10747
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher
• Application Notes: Functional Testing: order azide-free formatFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.