Optimal dilutions for each application to be determined by the researcher
Application Notes:
Functional Activity (Inhibition): antibody blocks EGF receptor activationinhibits A431 cell proliferationarrests tumor growth in vivoaffinity purificationIP: 1-2µg/500ug protein lysateFlow Cytometry: 0.5-1 ug/million cellsThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
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