Optimal dilutions for each application to be determined by the researcher
Application Notes:
Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
FACS surface staining of human PBMCs using CD45RA antibody (clone 158-4D3, red), and isotype control (green).
Flow cytometry testing of human Jurkat cells with CD45RA antibody (clone 158-4D3), Red=isotype control, Blue= CD45RA antibody.
IHC staining of FFPE human tonsil with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human spleen with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human spleen lysate with CD45RA antibody (clone 158-4D3). Expected molecular weight: 147-220 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone 158-4D3) as confirmation of integrity and purity.
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