MFGE8 Antibody [MFG-06], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: MFGE8 Antibody [MFG-06], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-33-436
• Supplier Catalog Number: 33-436
• Alternative Catalog Number: PRS-33-436-100
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC-P, WB
• Species Reactivity: Canine, Human
• Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.
• Conjugation: Unconjugated
• Alternative Names: Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1, Lactadherin short form, Medin, MFGE8

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL
• Clone Designation: [MFG-06]
• NCBI: 4240
• UniProt: Q08431
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher
• Application Notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Images

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFGE8 antibody (MFG-06)

FFPE human pancreas tested with MFGE8 antibody (MFG-06)

FFPE human lung carcinoma tested with MFGE8 antibody (MFG-06)

FFPE human renal cell carcinoma tested with MFGE8 antibody (MFG-06)

Western blot testing of MCF7 cell lysate with MFGE8 antibody (clone MFG-06).

SDS-PAGE Analysis of Purified, BSA-Free MFGE8 Antibody (clone MFG-06). Confirmation of Integrity and Purity of the Antibody.