Optimal dilutions for each application to be determined by the researcher
Application Notes:
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin antibody (B2M/961).
Formalin-fixed, paraffin-embedded human melanoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human renal carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin antibody.
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody.
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