Optimal dilutions for each application to be determined by the researcher
Application Notes:
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTWB: 0.5-1 ug/mlTitering of the Moesin antibody may be required for optimal performance.
IHC testing of FFPE human melanoma with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human placenta with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE rat colon with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE rat lung with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Western blot testing of human HT29 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/491). Confirmation of Integrity and Purity of the Antibody.
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