Bcl-2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Bcl-2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-3335
• Supplier Catalog Number: 3335
• Alternative Catalog Number: PRS-3335-0.02,PRS-3335-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-Bcl-2 antibody (3335) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2.
• Conjugation: Unconjugated
• Alternative Names: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 26kDObserved: 26 kD
• NCBI: 596
• UniProt: P10415
• Buffer: Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1-2µg/mL, IHC-P: 2 µg/mL, IF: 10 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human and mouse samples , Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Images

Figure 1 Western Blot Validation in Human and Mouse Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: Bcl-2 3335, (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells

Figure 5 Immunofluorescence Validation of Bcl-2 in Human Kidney CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Human Kidney Cells labeling Bcl-2 with 3335 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 7 Immunofluorescence Validation of Bcl-2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with 3335 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Figure 4 Immunohistochemistry Validation of Bcl-2 in Human Kidney Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (3335) at 2&956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of Bcl-2 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-Bcl-2 antibody (3335) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: Bcl-2 3335, (2 &956,g/mL), Bcl-2 3337, (2 &956,g/mL), beta-actin (1 &956,g/mL) and GAPDH (0.02 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: Bcl-2 3335, (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.