Avian Influenza Neuraminidase Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Avian Influenza Neuraminidase Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-3421
• Supplier Catalog Number: 3421
• Alternative Catalog Number: PRS-3421-0.02,PRS-3421-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Avian influenza neuraminidase antibody was raised against a synthetic peptide corresponding to 16 amino acids in the middle of the avian influenza neuraminidase protein.Efforts were made to use relatively conserved regions as the antigen.The immunogen is located within amino acids 150 - 200 of Avian Influenza Neuraminidase.
• Conjugation: Unconjugated
• Alternative Names: Avian Influenza Neuraminidase Antibody: Neuraminidase

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• UniProt: Q710U6
• Buffer: Avian Influenza Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL. Avian Influenza Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 µg/mL. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation with Avian Influenza NA Protein Coating Antigen: Avian Influenza Neuraminidase recombinant protein, 2 &956,g/mL, incubated at 4 &730,C overnight. Detection Antibodies: Avian Influenza NA antibody, 3421, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:10,000, incubated at RT for 1 hr.

Figure 1 Western Blot Validation with Avian Influenza NA Protein Loading: 50ng (Lane 1) and 100ng (Lane 2) Avian Influenza Neuraminidase recombinant protein. Antibodies: Avian Influenza antibody, 3421, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.