These newer COVID-19 nucleoprotein Mabs were developed using a recombinant, full-length nucleoprotein and then confirmed that they are reactive with the native virus itself.
Coronavirus Nucleocapsid Antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
Suggested starting ranges are 1:10-1:50 for IFA and 1:20-1:200 for ELISA. BEST ELISA pairs (capture-conjugate): clones 3865-3868 / 3869-3868
Figure 2 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid AntibodiesAntibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-721 and 35-722. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-721, 2ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-722, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng
Figure 1 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid AntibodiesAntibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-722 and 35-719. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-722, 2ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-719, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng
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