SARS-CoV Matrix Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV Matrix Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-3529
• Supplier Catalog Number: 3529
• Alternative Catalog Number: PRS-3529-0.02,PRS-3529-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2/SARS-CoV Matrix antibody (3529) was raised against a peptide corresponding to 15 amino acids near the carboxy-terminus of SARS-CoV Matrix protein. The immunogen is located within the last 50 amino acids of SARS-CoV Matrix.
• Conjugation: Unconjugated
• Alternative Names: E1 glycoprotein, M protein, Membrane protein, SARS Matrix protein, SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, sars-cov

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740571
• UniProt: O62114
• Buffer: SARS-CoV-2/SARS-CoV Matrix Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL.Antibody validated: SARS-CoV-2/SARS-CoV Matrix antibody can be used for the detection of SARS-CoV-2/SARS-CoV Matrix protein in ELISA. It will detect 5 ng of free peptide at 1 µg/mL.

Images

Figure 2 ELISA Test Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, 3529. A direct ELISA was performed using Matrix recombinant protein as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detection range is from 8ng/mL to 2000ng/mL.

Figure 3 ELISA Test Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, 3529 (1 &956,g/mL). A direct ELISA was performed using immunogen (3529P) as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000ng/mL.

Figure 1 Western Blot Validation with SARS-CoV-2 Matrix Recombinant ProteinLoading: SARS-CoV-2 Matrix recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Matrix (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 10 ng Matrix protein, Lane 2: 25 ng Matrix protein and Lane 3: 50 ng Matrix protein.