SARS-CoV-2 (COVID-19) Envelope Antibody (biotin), Biotin, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Envelope Antibody (biotin), Biotin, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-3531-BIOTIN
• Supplier Catalog Number: 3531-biotin
• Alternative Catalog Number: PRS-3531-BIOTIN-0.02,PRS-3531-BIOTIN-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody (3531) was raised against a peptide corresponding to 10 amino acids near the amino terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope.
• Conjugation: Biotin
• Alternative Names: 2019-nCoV) Envelope Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), E protein, Envelope protein, SARS-CoV-2 (COVID-19, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740570
• UniProt: QHD43418
• Buffer: SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: IHC: 2 µg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.

Images

Figure 1 ELISA Validation Coating Antigen: immunogen peptide, 3531P, 10 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 3531-biotin or 3531, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3531-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 3531 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.

Figure 2 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Envelope Protein in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) envelope antibody (3531-HRP, 2 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C, following by streptavidin-HRP conjugate at 20 &956,g/mL. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.